霍亂弧菌通用型核酸PCR檢測(cè)試劑盒

廣州健侖生物科技有限公司

廣州健侖長(zhǎng)期供應(yīng)各種流感檢測(cè)試劑，包括進(jìn)口和國(guó)產(chǎn)的品牌，主要包括日本富士瑞必歐、日本生研、美國(guó)BD、美國(guó)NovaBios、美國(guó)binaxNOW、凱必利、廣州創(chuàng)侖等。

產(chǎn)品名稱(chēng)：霍亂弧菌通用型核酸PCR檢測(cè)試劑盒；

 產(chǎn)品品牌：創(chuàng)侖；

 產(chǎn)品用途：本PCR-熒光探針?lè)?/span>體外定性檢測(cè)霍亂弧菌通用型核酸；

 產(chǎn)品規(guī)格：24T/盒； 

 保存條件：避光 -20度 保存。

    我司還提供各種流感病毒、副流感病毒、呼吸道合胞病毒、冠狀病毒、輪狀病毒、桿菌、鏈球菌、熱帶病毒等等核酸檢測(cè)試劑盒（PCR-熒光探針?lè)ǎ?/strong>，隨時(shí)歡迎您的來(lái)電

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【公司名稱(chēng)】 廣州健侖生物科技有限公司
【市場(chǎng)部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

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The principle and scope of application of Escherichia coli 0157 test tablets

1, principle and scope of application

Escherichia coli 0157 test piece is a pre prepared, disposable medium product, containing selective media and specific enzyme chromogenic agent, and colony on the test piece is blue, which can shorten counting time and enhance counting effect. This product is suitable for the determination of Escherichia coli 0157:H7 in all kinds of food and drinking water.

2. Methods of use

2.1, sample treatment: take sample 25mL (g) into the sampling tank or homogenized cup containing 225mL sterilized phosphate buffer diluent (or normal saline), make the 1:10 sample homogenate, and adjust the sample homogenization pH to 6.6 to 7.2 when necessary, with 1mol/LNaOH or 1mol/L HCl solution. Sterilize the pipette with 1mL, absorb the 1:10 sample and homogenize 1mL, pour into the test tube containing 9mL diluent, shake it up and become the sample of 1:100, homogenize it, and so on, and make the sample such as 1:1000 dilution to homogenize, and change one suction tube at a time.

2.2, inoculation: general food should be selected by 2~3 dilution tests. Liquid samples containing less bacteria (such as drinking pure water and mineral water) can be directly extracted from the original liquid for testing. The test piece was placed on the flat test table, the upper membrane was uncovered, the 1mL sample was collected by aseptic pipette, and the sample was uniformly distributed to the paper. The top layer was slowly covered and placed at 10s for about two pieces. At the same time, a blank negative control was made.

2.3, c*tion: put the test pieces together and put them back in the original self sealed bag and seal them. The transparent face is placed in the incubator with constant temperature, and the number of stacked pieces is not more than 12 pieces. The temperature of general food culture is 36? 1 C and 15 ~ 24h, and the culture temperature of aquatic products is 30 and 1 C, and the culture is 48h.

3, result interpretation

After culture, the purple red colonies on the test tablets were Escherichia coli 0157:H7, and the blue colony was the colony of other coliform colony. Samples of positive colonies are best verified by other more reliable methods, and at least repeat samples of the samples without conditions.