EIKEN食物感染流感病毒快檢卡（膠體金法）

廣州健侖生物科技有限公司

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EIKEN食物感染流感病毒快檢卡（膠體金法）

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【公司名稱】 廣州健侖生物科技有限公司
【市場(chǎng)部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

設(shè)立陽(yáng)性對(duì)照是病理醫(yī)生的任務(wù)或責(zé)任，而不是技術(shù)員的責(zé)任。病理醫(yī)生觀察了HE切片，了解切片中是否有自身對(duì)照，如果沒(méi)有，就應(yīng)告訴技術(shù)員采用陽(yáng)性對(duì)照。因此，病理醫(yī)生在免疫組化中的作用是不可忽視的。抗體未覆蓋上測(cè)試組織：當(dāng)多塊散開的小組織染色時(shí)，可能漏掉某塊組織染色。
二、“雜音”染色片
免疫組化除正常的真實(shí)的陽(yáng)性信號(hào)外常常會(huì)遇到不正常的背景著色，這些非正常的著色稱為“雜音”染色。“雜音”染色種類繁多，產(chǎn)生的原因也多種多樣，為了便于說(shuō)明，筆者將其歸納為下面幾種。
1、全片著色
全片著色是指整個(gè)切片全都染上了顏色，著色的強(qiáng)度可深可淺，總之，分不清那些組織是陽(yáng)性那些組織是陰性。出現(xiàn)這種現(xiàn)象的原因有：
（1）抗體濃度過(guò)高：一抗?jié)舛冗^(guò)高是常見(jiàn)的原因之一。解決辦法是，每次使用新抗體前應(yīng)當(dāng)對(duì)其工作濃度進(jìn)行測(cè)試，使每一抗體個(gè)體化，找到適合自己實(shí)驗(yàn)室的理想工作濃度，既使是即用型的抗體也應(yīng)如此，不能只簡(jiǎn)單的按說(shuō)明書進(jìn)行染色。
（2）抗體孵育時(shí)間過(guò)長(zhǎng)或溫度較高：解決辦法是，嚴(yán)格執(zhí)行操作規(guī)程，隨身佩帶報(bào)時(shí)表或報(bào)時(shí)鐘，及時(shí)提醒，避免因遺忘而造成時(shí)間延長(zhǎng)。現(xiàn)在流行的二步法（Polymer）敏感性很高，要求一抗孵育的時(shí)間不是傳統(tǒng)的1小時(shí)，而是30分鐘，因此，要根據(jù)染色結(jié)果進(jìn)行調(diào)整。

Setting up a positive control is the pathologist's task or responsibility, not the technician's responsibility. The pathologist looked at the HE section to see if there was a self-control in the section, and if not, l the technician to use a positive control. Therefore, pathologists in the role of immunohistochemistry can not be ignored. Antibodies are not covered by the test tissue: When multiple small stained tissues are stained, one tissue may be left missing.
Second, "murmur" stained film
Immunohistochemistry in addition to the normal real positive signals often encounter abnormal background coloring, these non-normal coloring called "murmur" staining. There are many kinds of "murmur" stains, which are caused by many reasons. For convenience, I summarize them as follows.
1, the whole film coloring
Whole-piece coloring refers to the entire section of the color dyed, the depth of the shading can be shallow, in short, can not l those organizations are positive those organizations are negative. The reasons for this phenomenon are:
(1) antibody concentration is too high: a high concentration of anti-one is a common cause. The solution is to test the working concentration before each use of the new antibody so that each antibody is individualized to find the ideal working concentration for your laboratory, even for ready-to-use antibodies, not just simple According to the instructions for staining.
(2) antibody incubation time is too long or high temperature: The solution is to strictly enforce the rules, it is best to wear timepieces or clock, timely reminder to avoid the delay caused by the forgotten. Now the popular two-step method (Polymer) high sensitivity, requiring a first antibody incubation time is not the traditional one hour, but 30 minutes, therefore, according to the staining results to be adjusted.