禽流感病毒H7亞型核酸PCR檢測試劑盒

廣州健侖生物科技有限公司

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禽流感病毒H7亞型核酸PCR檢測試劑盒

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室

物理性污染的預防
● 實驗室的合理設計及建立規(guī)范的操作規(guī)程；
● 培養(yǎng)箱應放在恒溫的環(huán)境中；
● 培養(yǎng)液及試劑應放在固定位置，而且要注意避光，試劑周圍不能放置同位素；
● 從冰箱中取出的培養(yǎng)液應在室溫條件中放置一段時間后再進行使用，以避免過冷的溫度對細胞的影響。
B.化學性污染的預防
● 使用的所有物質都應是高純度的，同時正確配制和儲存培養(yǎng)液及試劑。
● 在配制液體和清洗容器時必須使用不含雜質的超純水。
● 選用同一批次的血清。
● 在選用器皿時，根據被培養(yǎng)細胞的生長特性、培養(yǎng)方式和所用培養(yǎng)液的性質來選用適合的器皿來達到實驗的準確性和可靠性。
C.生物性污染的預防
● 建立規(guī)范的無菌操作程序及各種規(guī)章制度，并嚴格執(zhí)行。
● 控制環(huán)境污染，同時注意實驗人員的防護及無菌服的潔凈和無菌。
● 保證細胞培養(yǎng)基和器材無菌。
● 在細胞培養(yǎng)基中加入適量的抗生素。
預防是防止細胞培養(yǎng)過程中發(fā)生污染的辦法。只有提前做好預防工作，才能將發(fā)生
污染的可能性降到zui小程度。

Cell culture pollution refers to the invasion of elements which are harmful to the survival of cells and the foreign bodies that cause cell mutation in the culture environment. In vitro cell culture pollution can be divided into three categories: physical, chemical and biological pollution.

Physical pollution:
Physical pollution affects the metabolism of cells by affecting the biochemical components of the cell culture system.
The sources of physical pollution: physical factors in the culture environment, such as temperature, radiation, vibration, radiation (ultraviolet or fluorescence), can affect cells.
The phenomenon of physical pollution: cells, culture fluids or other culture reagents exposed to radiation, radiation or supercooled superheated temperatures can cause changes in cellular metabolism, such as cell synchronization, cell growth inhibition, and even cell death.
Chemical pollution
Chemical pollution is a chemical substance that is toxic to cells or stimulates cells.
Sources of chemical pollution:
Containers of non purified substances, reagents, water, serum, growth cofactors and storage reagents can all be the source of chemical pollution. The essential nutrients (such as amino acids) in cell culture, if the concentration exceeds the reasonable range, can also be toxic to the cells. In the same way, the requirements of different cell lines to the serum and buffer solution are different under the best culture conditions, and should be strictly controlled in the culture.
Chemical pollution: chemical pollution can also inhibit cell growth and even cell death.
Biological pollution
Biological pollution includes contamination of bacteria, fungi, and yeast, which are easier to detect, and the contamination of viruses, mycoplasma and other cells that are more difficult to detect.
Sources of biological pollution:
Inadvertently or mishandled
Air dust
Disinfection is not strict
Improper operation
Reagents, especially serum pollution
The phenomenon of biological pollution:
Bacteria: more G+ bacteria.
Fungi: more fungi.
Mycoplasma: it is not easy to detect.
Virus: the virus has a species specificity, so the probability of virus contamination is relatively small.