丹麥SSI沙門氏菌診斷血清

注意：丹麥SSI診斷血清2018年已漲價

沙門氏菌Vi血清（SSI沙門表面抗原血清）

Vi抗原介紹

表面抗原（即Vi抗原）是一種特殊的菌體抗原，屬于K抗原群。

因與毒力有關而命名為Vi抗原。

由聚-n-乙酰-d-半乳糖胺糖醛酸組成。

不穩定，經60°加熱，石碳酸處理或人工傳代培養易破壞或丟失。

Vi抗原存在于細菌表面，可阻止O抗原與其相應抗體的反應。

Vi抗原的抗原性弱，當體內菌存在時可產生一定量抗體；當細菌被清除后，抗體也隨之消失。

Vi抗原血清目錄

沙門氏菌Vi血清（SSI沙門表面抗原血清）

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沙門氏菌的抗原分為菌體抗原(O)、鞭毛抗原（H）、莢膜抗原（K、Vi）、纖毛抗原。O抗原O抗原存在于菌體細胞壁zui外層，化學成分為類脂-多糖-多肽復合物，由多糖決定其特異性。O抗原耐熱、醇和酸，100～121℃加熱2.5h、用乙醇或鹽酸處理而不失去抗原性，因此菌體抗原已經被*為沙門氏菌血清型分型的基礎。一種菌體有一種或多種不同的O抗原。沙門氏菌的O抗原共有65種，以阿拉伯數字1、2、3……表示。主要抗原：O抗原為某一菌群*而其他菌群不具有。如：2、3、4、7、8、9、10等。次要抗原：O抗原為幾個菌群共有。如：1、5、6、12等。 H抗原存在于鞭毛之中，為蛋白質，由肽鏈中氨基酸的排列順序及空間構型決定其特異性。不耐熱，經加熱和用乙醇及堿處理易變性。H抗原常有兩相的變異。*相為特異相，用小寫英文字母表示，如：a、b、I、e、h等；第二相為非特異相，用阿拉伯數字表示，如：1、2、5等，但也有少數菌含有*相中的抗原e、n、x等成分。 為O抗原表面的莢膜抗原。具有該抗原的細菌，由于可阻止O抗原與抗體結合，因此不被相應的O血清凝集。60℃加熱處理或石炭酸處理，雖然該抗原不被滅活，而已從菌體表面脫落，游離于液體中，從而暴露出O抗原，可被O血清凝集。要進行O凝集反應，必須先洗掉Vi抗原。 纖毛抗原是一種比鞭毛更細小的纖維樣結構物，含有蛋白性的抗原物質。纖毛能凝集豚鼠的紅細胞。所有沙門氏菌的纖毛抗原性一樣。纖毛的存在不影響活菌的凝集性，但含有極豐富的纖毛抗原的菌株，有時也組織O的凝集性。

Salmonella antigen is divided into O, flagellum antigen (H), capsular antigen (K, Vi) and cilia antigen. The O antigen of O antigen is found in the outer layer of cell wall. Its chemical composition is lipid polysaccharide polypeptide complex, and its specificity is determined by polysaccharides. O antigen is heat-resistant, alcohol and acid. It is heated for 2.5h at 100~121 C and is treated with ethanol or hydrochloric acid without losing its antigenicity. Therefore, the bacterial antigen has been recognized as the basis of serotype classification of Salmonella. A fungus has one or more different O antigens. There are 65 kinds of O antigens of Salmonella, the number is 1, 2, 3 in Arabia. Express。 The main antigen is O antigen, which is specific for a certain flora but not for other bacteria. Such as: 2, 3, 4, 7, 8, 9, 10, etc. Secondary antigen: O antigen is a group of bacteria. Such as: 1, 5, 6, 12 and so on. H antigen exists in flagellum, which is protein. Its specificity is determined by the sequence and spatial configuration of amino acids in peptide chains. It is heat resistant, heated and treated with ethanol and alkali. H antigen often has two phases of variation. The first phase is a specific phase, which is expressed in lower case English letters, such as a, B, I, e, h, etc.; the second phase is a non specific phase, which is expressed in Arabia numbers, such as 1, 2, 5, etc., but a few bacteria contain the antigens of E, N and X in the first phase. A capsule antigen on the surface of O antigen. The bacteria with this antigen can not bind to the corresponding O serum because it can prevent the binding of O antigen to antibody. Heat treatment at 60 C or carbonic acid treatment, although the antigen is not inactivated, it is removed from the surface of the fungus and free in the liquid to expose the O antigen, which can be agglutinated by the O serum. In order to carry out O agglutination, we must first wash away the Vi antigen. Cilia antigen is a fibrous structure smaller than flagellum, which contains protein antigenic substances. Cilia can agglutinate the red blood cells of the guinea pig. The cilium of all Salmonella is antigenicity. The presence of cilia does not affect the agglutination of live bacteria, but the bacteria that contain very rich cilia antigen sometimes also organizes the agglutination of O.